Esterase and Lipase Activity of Hepatic Triglycerice Lipase in Post Heparin Plasma

A comparison of molecular properties of hepatic triglyceride lipase and lipoprotein lipase from human post-heparin plasma.

Hepatic triglyceride lipase was isolated from human post-heparin plasma by the method of Ehnholm et al. using modifications which increased the specific activity 12-fold to approximately 3,000 mumol of free fatty acid/h/mg of protein. Lipoprotein lipase with similar specific activity was prepared from the same plasma samples using heparin and concanavalin A affinity chromatography. The molecula...

Studies of fat lipolysis by post-heparin human plasma lipoprotein lipase and by human pancreatic lipase.

Body mass index and hepatic lipase gene (LIPC) polymorphism jointly influence postheparin plasma hepatic lipase activity.

The -514 polymorphism in the hepatic lipase gene (LIPC) is associated with decreased hepatic lipase activity. In the present study, the interaction between body mass index (BMI), the -514 polymorphism, and hepatic lipase activity was examined in 118 white men and in 51 African American men. BMI was significantly positively correlated with hepatic lipase activity in both populations. BMI was sim...

Effect of estrogen on post-heparin lipolytic activity. Selective decline in hepatic triglyceride lipase.

The rise in plasma triglyceride (TG) levels associated with estrogen administration has been thought to arise from impaired clearance because of the uniform suppression of post-heparin lipolytic activity (PHLA). Recently PHLA has been shown to consist of two activities: hepatic TG lipase and extrahepatic lipoprotein lipase (LPL). To determine whether estrogen might induce a selective decline in...

Measurement of lipoprotein lipase activity in post heparin plasma: description of technique*

S U M M A R Y A method has been described for the quantitative assay of lipoprotein lipase activity in post heparin plasma. The conditions of the away were subjected to critical study. The technique described permits the react.ion to proceed in the test tube as a zero order reaction. It is believed that this technique will permit accurate quantitative study of lipoprotein lipase activity in dif...